Hatha yoga, the valuable heritage of India teaches the art of attaining an perfect interacting activities in combination with mind, body, and energy. It has proven to have many therapeutic benefits. This study investigated the effect of hatha yoga with music on personal stress of female secondary school teachers. The study was a true experimental research. A sample of 120 female secondary school teachers was selected randomly from the government schools of Chandigarh. Total sample consisted of two groups (Experimental group - Hatha yoga with music and Control group - Non-hatha yoga with music) comprising of 60 female secondary school teachers each. Experimental group was given the treatment of hatha yoga with music whereas control group was not given any treatment. Then, the total group was administered Singh's Personal Stress Source Inventory. Analysis of results indicated a significant difference in personal stress of female secondary school teachers not practicing and practicing hatha yoga with music and revealed the effect of practice of hatha yoga with music on personal stress among school teachers. Findings of the study further helps improve the health ability and balance by reducing stress, benefitting in enhancing the quality of life of female secondary school teachers. Therefore, proves to be helpful for students, teachers, parents and educationists by helping them to connect between mind and body with the help of hatha yoga with music and achieve a greater level of reflection and relaxation.